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Bioingenium

Mammalian expression

Producing proteins in mammalian cells

CHO and HEK cells are the most stablished cell lines for production of mammalian complex proteins. Both cell lines are able to secrete proteins to culture medium where the protein of interest could be recovered.

Benefits:

  • Transient transfection
  • Stable cell lines
  • Production of recombinant antibodies
  • GMP banked cell lines


Our s
ervices:

Preliminary protein production study in CHO and HEK

Prior to perform transfection at higher cultivation volumes we recommend to carry out a small scale preliminary production study in CHO and HEK to assess the best cell line for your protein of interest. That would save you time and resources and would increase the chances to obtain the maximal protein productivity. In this study we will analyze in parallel both cell line and also will perform a time course analysis of the production.

  • Gen synthesis and cloning
  • Transient transfection with CHO and HEK for determination of the best production cell line
  • Time course analysis of production for CHO and HEK
  • Cultivation at 50 mL volume in serum free medium
  • Analysis by SDSPAGE and western blot
  • Endotoxin analysis (optional)

Deliverables: purified protein and certificate of analysis


Protein production in CHO and HEK

Our transient mammalian expression service offers fast production of high quality proteins produced in mammalian cells, from milliliter to liter scale.

  • Gen synthesis and cloning
  • Transient transfection with CHO or HEK cells
  • Cultivation at 500 mL, 1000 mL, 5000 mL volume in serum free medium. For higher culture volumes contact us.
  • Purification by affinity chromatography
  • Analysis by SDSPAGE and western blot
  • Endotoxin analysis (optional)
Deliverables: All purified protein from specified culture volume (alternatively, culture supernatant or cells)


Stable cell line development


For more information or to request a quote about stable cell line development, please contact us.


Quality certification: