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Bioingenium

Pichia pastoris expression

Producing proteins in Pichia pastoris

The advantages of Pichia pastoris (P. pastoris) as expression system are well know: it has the ability to perform post-translational modification like disulphide bridges, and also has the microbial capacity to growth in inexpensive media up to very high cell densities. On the other hand, P. pastoris is able to secrete the recombinant protein into the culture medium, simplifying the recovery of the protein if interest.

At Bioingenium we have a large experience in recombinant protein expression in P. pastoris system and 20 scientific papers published.

Bioingenium has an extensive collection of proprietary vectors designed to increase production titers and to simplify the cloning and expression process. 

Benefits:

  • High yield and low productions costs for human proteins
  • Removable resistance markers genes
  • Inducible AOX1 and constitutive GAP promoter
  • Multi-copy vectors
  • Improved strains by co-expression of chaperones
  • Fermentation process development


Our feasibility studies
:

  • Gene synthesis and cloning
  • Test improved strain co-expressing chaperones
  • Evaluation of alternative promotors (AOX vs GAP)
  • Screening of hundreds of Pichia clones
  • Screening of culture conditions to maximize productivity
  • Analysis by SDS-PAGE and Western Blot
  • Purification by affinity chromatography or standard chromatographic techniques. 
Deliverables: All purified protein from specified culture volume (alternatively, culture supernatant or cells)

Our protein production in bioreactor:

  • High cell density fermentation in bioreactor
  • Fermentation volume 5 L, 50 L to 300 L
  • Analysis by SDS-PAGE and Western Blot
Deliverables: All purified protein from specified culture volume (alternatively, culture supernatant or cells). For more information please contact us.